Effects of silencing lncRNA SLC16A1-AS1 on malignant biological behaviors of brain glioma cells
LONG Yin-bo
LI He-yang
JIN Zhi-bin
Abstract：Objective To investigate the effects of silencing long non-coding RNA(lncRNA)SLC16A1-AS1 on the malignant biological behaviors of glioma cells.Methods LncRNA SLC16A1-AS1,miR-584-5p and MAPK1 mRNA were detected by PCR in glioma tissues obtained from 62 patients who underwent surgery from May 2021 to January 2023,and MAPK1 protein expression was detected by Western blotting.Para-tumor tissues(>2 cm from the edge of the tumor)were used as control.Glioma cells were isolated and cultured from the glioma tissues,and CD133 and Neatin were identified by immunofluorescence staining.Different plasmids were transfected to silence lncRNA SLC16A1-AS1,up-regulate or down-regulate the expression of miR-584-5p.CCK-8 method,scratch experiment,Transwell experiment and flow cytometry were used to detect the proliferation,migration,invasion and apoptosis of the cultured glioma cells.The protein expressions of MAPK1,CyclinD1,MMP-2 and caspase-3 were detected by Western blotting.The dual luciferase reporter gene assay was used to verify the targeting relationship between lncRNA SLC16A1-AS1,miR-584-5p and MAPK1.Results LncRNA SLC16A1-AS1 and MAPK1 were significantly highly expressed in glioma tissues(P<0.05),and miR-584-5p was significantly lowly expressed(P<0.05).The immunofluorescence staining showed that the isolated and cultured cells were positive for CD133 and Nestin.Silencing of lncRNA SLC16A1-AS1 significantly inhibited the proliferation,migration and invasion of the glioma cells in vitro(P<0.05),promoted cell apoptosis(P<0.05),significantly down-regulated the protein expressions of CyclinD1,MMP-2 and MMP-9(P<0.05),and significantly up-regulated the expressions of miR-584-5p and caspase-3.Inhibition of miR-584-5p significantly reversed the effects of lncRNA SLC16A1-AS1 silencing on the glioma cells in vitro.Dual luciferase reporter gene assay confirmed that lncRNA SLC16A1-AS1 was targeted to regulate miR-584-5p/MAPK1.Conclusions LncRNA SLC16A1-AS1 is highly expressed in glioma tissues.Silencing lncRNA SLC16A1-AS1 can up-regulate the expression of miR-584-5p and inhibit the expression of MAPK1,thereby inhibiting the proliferation,migration and invasion of glioma cells and promoting cell apoptosis.
Keywords：Brain gliomaLong non-coding RNA SLC16A1-AS1miRNA-584-5pMAPK1Malignant biological behaviors
Publication Date：2024-01-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 35-41,45 )