Effects of hyperoside on inflammatory response and blood-brain barrier permeability in rats after traumatic brain injury

QIU Hui-bin; JIANG Jin-li; LI Peng-qiang; SHAN Chun-ge; WANG Chao

doi:10.13798/j.issn.1009-153X.2024.01.009

Abstract：Objective To investigate the effects of hyperoside on inflammatory response and blood-brain barrier(BBB)permeability in rats after traumatic brain injury(TBI)and its underlying mechanisms.Methods Sixty adult SPF SD rats were randomly divided into sham operation group,model group,low-dose hyperoside group,high-dose hyperoside group,lipopolysaccharide(LPS)group,and high-dose hyperoside+LPS group,with 10 rats in each group.The TBI model was established by modified Feeney free fall method.Rats in the low-dose and high-dose hyperoside groups were given hyperoside solution by gavage after modeling,with doses of 60 and 120 mg/kg,respectively;rats in the LPS group were given LPS solution by gavage after modeling,with a dose of 0.4 mg/kg;rats in the hyperoside+LPS group were given hyperoside solution and LPS solution by gavage after modeling;once a day for 14 days.At 24 h after gavage,neurological function was evaluated by modified neurological severity scale(mNSS)score,cognitive function was detected by the platform jumping test,BBB permeability was detected by Evans blue(EB)quantitative method,structural damage of BBB was observed by transmission electron microscopy,levels of inflammatory mediators[tumor necrosis factor(TNF-α),interleukin-17(IL-17),inducible nitric oxide synthase(iNOS)]in serum and brain tissues were measured by ELASA,and protein expression levels of TNF-α/NF-κB/caspase-3 in brain tissue were detected by Western blotting.Results After TBI,the mNSS score and the platform latency were significantly decreased(P<0.05),while the number of platform errors,brain EB content,serum and brain inflammatory mediators,brain TNF-α and caspase-3 protein expression,and p-NF-κB p65/NF-κB p65 were significantly increased(P<0.05);LPS significantly aggravated the neurological injury of TBI rats(P<0.05),significantly increased the levels of inflammatory mediators(P<0.05),and significantly increased the TNF-α/NF-κB/caspase-3 protein expression(P<0.05);hyperoside significantly improved the brain injury of TBI rats(P<0.05),with a dose-dependent manner;high dose hyperoside significantly reversed the effects of LPS(P<0.05).Conclusions Hyperoside can reduce the BBB injury and improve the neurological function of TBI rats by inhibiting the TNF-α/NF-κB/caspase-3 signaling pathway and inhibiting inflammatory response.