Relationship between H3K27me3 and survival prognosis of patients with high-grade gliomas

CHEN Zhi-chao; XUE Ao-nuo; SHI Jin-zhao

doi:10.13798/j.issn.1009-153X.2023.10.008

Abstract：Objective To investigate the relationship between expression of trimethylated histone H3 at lysine 27(H3K27me3)in glioma tissues and survival prognosis of patients with high-grade gliomas.Methods The expressions of H3K27me3 were detected in glioma tissues obtained from 64 patients with high-grade gliomas who underwent surgery from June 2015 to September 2017,and in non-tumor brain tissues obtained from 10 patients with traumatic brain injury who underwent decompression using immunohistochemical staining.The protein expression of H3K27me3 was detected by western blotting in non-tumor brain tissues(n=3),WHO grade Ⅲ glioma tissues(n=3)and WHO grade Ⅳ glioma tissues(n=3).Patients with gliomas were followed up until October 2022 and their overall survival times were recorded.Results Immunohistochemical staining showed that the high expression rate of H3K27me3 in high-grade glioma tissues(59.37％)was significantly higher than that(20.00％)in non-tumor brain tissues(P<0.05).Western blotting showed that H3K27me3 protein expression level in WHO gradeⅣglioma tissues was significantly higher than those in WHO grade Ⅲ glioma tissues and non-tumor brain tissues(P<0.05).The median follow-up time of 64 patients with glioma was 62.23 months,and the 5-year survival rate was 37.5％.Multivariate Cox regression analysis showed that high expression of H3K27me3 was an independent risk factor for poor survival prognosis of patients with high-grade gliomas(P<0.05).Survival curve analysis showed that the median survival time(18 months)of patients with high H3K27me3 expression was significantly lower than that of patients with low H3K27ME3 expression(33 months;P<0.05).Conclusions H3K27me3 is highly expressed in high-grade gliomas,which is associated with poor survival prognosis of patients.