Effects of Xingnaojing injection on early brain injury in rats after subarachnoid hemorrhage

JIAO Ji-chao; SUN Lin-lin

doi:10.13798/j.issn.1009-153X.2023.09.011

Abstract：Objective To investigate the effects of Xingnaojing injection on early brain injury(EBI)in rats after subarachnoid hemorrhage(SAH).Methods Thirty-six adult male SD rats were randomly divided into control group,model group,and Xingnaojing group,with 12 rats in each group.The SAH model was established by puncturing the internal carotid artery.The control group did not undergo vascular puncture,while the rest of the procedures were the same as in the model group.After successful modeling,the rats in the Xingnaojing group received intraperitoneal injection of Xingnaojing injection(10 ml/kg/d),while the rats in the model and control groups received an equal amount of normal saline.At 24 hours post-modeling,neurological function of rats was evaluated using the Garcia JH score,brain water content was assessed using the wet-dry method,IL-1β and IL-18 levels in the hippocampal tissues were measured by ELISA,and the expression levels of NLRP3 and Caspase-1 in the hippocampal tissues were detected by immunohistochemistry and immunoblotting.Results Compared to the control group,the rats in the model group showed a significant decrease in Garcia JH score(P<0.05),a significant increase in brain water content(P<0.05),and a significant increase in IL-1β,IL-18,NLRP3,and Caspase-1 levels in the hippocampal tissues(P<0.05).Compared to the model group,the rats in the Xingnaojing group had a significant increase in Garcia JH score(P<0.05),a significant decrease in brain water content(P<0.05),and a significant decrease in IL-1β,IL-18,NLRP3,and Caspase-1 levels in the hippocampal tissues(P<0.05).Conclusion Xingnaojing injection can improve EBI in rats after SAH,possibly by reducing the level of neuronal pyroptosis.