Resveratrol inhibits tumor growth in nude mice model of glioma
HU Sen
ZHANG Hui
BU Xingyao
FENG Miaomiao
WANG Bangqing
WANG Junyi
YANG Bin
YANG Huachao
Abstract：To investigate the effect of resveratrol (RES) on tumor growth in nude mice model of glioma. Methods Eighty nude mice models of glioma were established by injection of U87 cells into the cerebral tissues, then these nude mice were randomly divided into RES intervention group (administration 10 days after injection of U87 cells, 40 mg/kg) and solvent control group (administration 10 days after injection of U87 cells, once a day, 10 mg/kg; the solvent was 0.5% sodium carboxymethyl cellulose). According to the route of administration, the RES intervention group was then divided into RES intragastric group and RES transnasal group, and the solvent control group was divided into solvent intragastric group and solvent transnasal group, with 20 mice in each group. The survival time was analyzed by Kaplan-Meier method, the tumor volume was measured at 14, 21, 28 and 35 days after injection of U87 cells, the microvessel density (MVD) was calculated by labeling the vascular endothelial cells with CD31, the expressions of vascular endothelial growth factor (VEGF) and Ki-67 were detcted by immunohistochemical staining, and the apoptosis of glioma cells was detected by TUNEL method. Results Compared with the solvent control group, the survival time of nude mice was significantly prolonged (P<0.05), the tumor volume was significantly reduced (P<0.05), the MVD of tumor tissue was significantly decreased (P<0.05), the expressions of VEGF and Ki-67 in tumor tissue was significantly decreased (P<0.05), and the apoptosis rate of tumor cells was significantly increased in RES intervention group (P<0.05). Moreover, the tumor inhibitory effect of RES by transnasal administration was significantly better than RES by intragastric administration (P<0.05). Conclusion RES can effectively inhibit the tumor growth in nude mice model of glioma, which may be related to the inhibition of tumor angiogenesis and the promotion of tumor cell apoptosis. Compared with the intragastric administration, transnasal administration of RES has better tumor inhibitory effect.
Keywords：GliomaResveratrolNude mice model of gliomaTumor angiogenesisTumor growthTumor cell apoptosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 543-546 )