Effects of apigenin on proliferation, invasion and migration of glioma cell line U87 cells

XIAO Qing-bao; CHEN Bo; YU Xiao-xiang

doi:10.13798/j.issn.1009-153X.2019.08.012

Abstract：Objective To explore the effects of apigenin on the proliferation, invasion and migration of glioma cell line U87 cells and their mechanism. Methods U87 cells were cultured in vitro and then were divided into blank control group, experimental group I in which U87 cells were treated with low-dose apiginen (20 μmol/L), experimental groupⅡin which the cells were treated with medium-dose apigenin (40 μmol/L) and experimental groupⅢin which the cells were treated with high-dose apigenin (80 μmol/L). The U87 cells proliferation, apoptosis, and invasive and migration abilities were determined respectively by MTT, Hocst staining, transwell test and scratch assay. The levels of matrix metalloproteinase (MMP)-2, MMP-9, Recombinant Bcl2 Antagonist/Killer 1 (Bak1), cysteinyl aspartate specific proteinase-3 (Caspase-3) expressions in the U87 cells were detected by Western blotting. Results Compared with the blank control group, the abilities of U87 cells proliferation, migration and invasion were significantly 1nhibited in all the experimental groups (P<0.05). The rates of the inhibition of U87 cells proliferation, migration and invasion were positively related to the dose of apigenin used to treat the U87 cells (P<0.05). Compared with the blank control group, in the U87 cells the expressions of MMP-2, MMP-9 and Bak1 in the U87 cells were significantly inhibited and the protein expression of Caspase-3 protein was significantly up-regulated in all the experimental groups (P<0.01), The levels of MMP-2, MMP-9 and Bak1 protein expression in the U87 cells were negatively related to the dose of apigenin used to treat the U87 cells and the level of Caspase-3 protein expression in the U87 cells was positively related to the dose used to treat the U87 cells (P<0.01). Conclusion It is suggested that apigenin can inhibit the proliferation, invasion and migration of glioma cell line U87 cells possibly by the down-regulation of the levels of MMP-2, MMP-9 and Bak1 protein expression and up-regulate the level of Caspase-3 protein expression.