Observation of effects of cortical spreading depression on cortical microcirculation after subarachnoid hemorrhage under transparent cranial window
SHE DEyuan
TANG Yue
HUANG Qin
WEN Jianpeng
LIU Peng
Abstract：Objective To observe the effects of cortical spreading depression (CSD) on the cortical microcirculation after subarachnoid hemorrhage (SAH). Methods Thirty C57 male mice were randomly divided into 3 groups of 10 animals each, i. e. normal group in which the transparent cranial windows were made and KCl dropped into the cranial window, control group in which the transparent cranial windows and SAH models were made and the physiological saline dropped into the window and experimental group in which the transparent cranial windows and SAH models were made and KCl dropped into the window. CSD and the cortical microcirculation was recorded by laser speckle blood flow imaging and endogenous signal imaging technology. Results①CSD appeared in the experimental group and the normal group, but not in the control group. ②Compared to those before dropping the reagent, the cerebral cortical blood flow significantly decreased (P<0.05) and the cortical blood vessels significantly constricted, especially the 5th day after SAH in the experimental group (P<0.05), the cortical blood vessels significantly dilated (P<0.05) and the cortical blood flow significantly increased (P<0.05) in the normal group and the cortical blood vessels and blood flow were insignificantly changed in the control group (P>0.05) after dropping the reagent. Conclusions①KCl can induce CSD in the normal and SAH mice cortexes.②CSD can induce the increase in the cerebral blood flow and vasodilatation in normal mice cortexes.③CSD can induce the decrease in the blood flow and vasoconstriction in the SAH mice cortexes.
Keywords：Subarachnoid hemorrhageCortical spreading depressionLaser speckle technologyEndogenous signal imagingCerebral blood flow
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 352-355 )