Effect of targeted silencing Wip1 gene on glioma cells proliferation and sensitivity to radiotherapy

YIN Lei; WANG Peng; HOU Chong-xian; LI Yan-wen; ZHOU Dong

doi:10.13798/j.issn.1009-153X.2018.05.011

Abstract：Objective To investigate the effects of targeted silencing of Wip1 gene on glioma cells proliferation and sensitivity to radiotherapy.Methods Glioma U251 cells which were cultured were transfected with Wip1 RNAi lentiviral vector.Then the U251 cells were treated with ionizing radiation (IR) and divided into negative control (NC) group, IR group, targeted silencing Wip1 gene (Wip1) group and IR+Wip1 group.The proliferation activities of U251 cells,Chk1 and Chk2 mRNA expressions and Chk1 and Chk2 protein expressions were detected respectively by CCK-8 kit,real time PCR and Western-blot in all the groups.Results The proliferation rates in the Wip1 and the IR groups were significantly lower than that in NC group 24,48,72,96 and 120 hours after the radiotherapy(P<0.05).The proliferation rate was significantly lower in the IR+Wip1 group than those in all the other groups 24,72,96 and 120 hours after the radiotherapy(P<0.05).The level of chk1 mRNA expression was significantly lower in IR+Wip1 group than that in Wip1 group and higher than those in IR and NC groups 24 hours after the radiotherapy (P<0.05). The level of chk2 mRNA expression was significantly lower in IR+Wip1 group than those in the other three groups (P<0.05).The level of Chk1 protein expression was significantly lower in IR+Wip1 group than those in IR and NC groups 24 hours after the radiotherapy(P<0.05).The level of Chk2 protein expression was significantly lower in the IR+Wip1 group than those in the other three groups(P<0.05).Conclusions It is suggested that targeted silencing Wip1 gene can inhibit the proliferation of glioma cells and may increase the sensitivity of glioma cells to radiotherapy by inhibiting the protein expressions of Chk1 and Chk2.