Effect of OCpG-ODN on cerebral tissues injured by cerebral ischemia/reperfusion and JAK and STAT expressions in rats

ZHENG Bo; CHEN Tao; MAO Hua

doi:10.13798/j.issn.1009-153X.2018.03.012

Abstract：Objective To explore protective effect of oCpG-ODN on cerebral tissues injured by cerebral ischemia/reperfusion(I/R)and the expressions of Janus kinase(JAK)and signal transduction and activator of transcription(STAT)in rats.Methods Brain I/R model was constructed by middle cerebral artery occlusion(MCAO)method.Fifty-four SD rats were randomly divided into three groups of 18 animals each,i.e.sham operation group,brain I/R group,and CpG-oligodeoxynucleotide(CpG-ODN)treatment group,in which 10 μg/25g CpG-ODN was intraperitonealy injected before the MCAO.Neurological function,cerebral infarction volume and morphological changes in the cerebral tissues were determined.The cells apoptosis and JAK and STAT mRNA levels and JAK,phosphorylated JAK, p-JAK, STAT, p-STAT and Caspase 3 and 7 proteins expressions in the infarct cerebral tissues were detected 24 hours after I/R. Results The cerebral dysfunction was significantly more severe and the volumes of the cerebral infarction was significantly bigger in the I/R group than those in CpG-ODN treatment group(P<0.05),which were significantly more severe and bigger than those in the sham operation group(P<0.05).The cell apoptosis rate,Caspase 3 and 7 proteins levels,the expression levels of mRNA of STA and JAK,and the levels of p-STA and p-JAK protein expressions were significantly higher in I/R group than those in the sham operation group and CpG-ODN treatment group. The levels of p-STA and p-JAK proteins expressions were significantly higher in CpG-ODN treatment group than those in the sham operation group (P<0.05). Conclusion It is suggested that CpG-ODN can effectively protect injured cerebral tissues after I/R possibly by promoting JAK and STAT protein phosphorylation and inhabiting cell apoptosis.