Effects of c-fos targeted silence on proliferation and invasiveness of human glioma cell U87

GUI Zhi-yong; FENG Jun; HUANG Jun-hong; BAI Jingyang

doi:10.13798/j.issn.1009-153X.2017.12.011

Abstract：Objective To explore the effects of lentivirus-mediated c-fos targeted silence on the proliferation and invasiveness of human glioma cell U87 in vitro. Methods Lentiviral vector c-fos-shRNA was transfected into human glioma U87 cells. Empty vector-transfected human glioma U87 cells served as the no-load control, and non-transfected human glioma U87 cells serve as the blank control.The morphology of U87 cells,the expression of c-fos mRNA and c-fos protein,the invasiveness and migration ability of U87 cells,and the survival rate of glioma cells,and the apoptosis rate of U87 cells were observed in all the groups.Results No green fluorescence of U87 cells in the blank control group,strong green fluorescence and clear cell outline of U87 cells in the no-load group and the transfection group were observed by the fluorescence microscope. The levels of c-fos mRNA and protein expressions were significantly lower in the transfection group 48 hours after the transfection than those in the no-load and blank control groups(P<0.05) The survival rate, migration and invasion ability of U87 cells were significantly lower in the transfection group 48 hours after the transfection than thsoe in the blank control and no-load groups (P<0.05). The number of apoptotic cells was significantly more in transfection group 72 hours after the transfection than that in the blank control and no-load groups (P<0.05). Conclusions It is suggested that c-fos targeted silence can significantly inhibit the proliferation and invasiveness of human glioma U87 cells, and c-fos-shRNA lentivirus-mediated gene therapy may be targeted as a new candidate for c-fos gene therapy.