Preliminary study of construction of eukaryotic over expression vector of arhgap39 gene and its protein expression in NG-108 cells
HUANG Chao-shuai
FENG Dong-fu
Abstract：Objective To construct eukaryotic overexpression vector of arhgap39 gene and to study the related characteristic of arhgap39 protein. Methods The total RNA of mouse hippocampus was extracted and cDNA was synthesized by reverse transcription PCR. The coding sequence of arhgap39 gene was amplified by high fidelity PCR according to the gene information of Gene Bank and then it was transferred to eukaryotic vector and transfected into NG-108 cells to observe its expression in the cells. Results The restriction enzyme digestion and sequencing showed that the eukaryotic over expression vector of arhgap 39 gene was successfully constructed. The eukaryotic over expression vector was transfected into NG-108 cells and Western blotting showed that the arhgap39 protein was over expressed in NG-108 cells. Conclusion The eukaryotic over expression vector of arhgap39 is successfully prepared. The molecular weight of arhgap39 protein is about 140 kD.
Keywords：Arhgap39 geneOver expression vectorShearMolecular weight
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 703-706 )