Effect of exogenous TRAIL gene transfection combined with chloroquine on glioma U251 cells apoptosis
FENG Chi
GUO Shuang-yi
CHENG Long-hai
LUO Jie
Abstract：Objective To investigate the effect of the exogenous tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and chloroquine (CQ) on the apoptosis of glioma U251 cells. Methods Human glioma U251 cells were treated with exogenous green fluorescent protein (GFP)-TRAIL and/or CQ. The expression level of GFP-TRAIL in U251 cells was detected by confocal microscope. The cells vitality was detected by MTT assay and U251 cells apoptosis was detected by Annexin V-FITC/PI Apoptosis Detection Kit. The expression levels of proteins related to apoptosis were detected by Western blot. Results GFP-TRAIL was stably expressed in U251 cells and induced the U251 cells apoptosis after the transfection of exgenous TRAIL into U251 cells. The rate of inhibition of U251 cells proliferation (47.22 ± 0.15)%and the U251 cell apoptosis rate (41.62 ± 0.44)%were significantly higher in the glioma U251 cells treated with GFP-TRAIL combined with CQ respectively than those [(23.88±0.22)%and (22.48±0.43)%respectively] in the glioma U251 cells only treated with GFP-TRAIL or CQ and control group (P<0.05). Conclusion The GFP-TRAIL protein can be stably expressed in U251 cells transfected with TRAIL gene and induce the apoptosis of U251 cells. GFP-TRAIL combined with CQ can enhance the apoptosis of U251 cells.
Keywords：GliomaU251 cellsApoptosisTumor necrosis factor-related apoptosis-inducing ligandChloroquine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 563-565 )