Expression of miRNA-221 in brain gliomas and its effects on glioma cell line poliferation
WEI Chuan-jiang
LIU Xian
WANG Ce
DONG Bai-jing
CHANG Ying-nan
ZHANG Yao
ZHAO Tian-shu
Abstract：Objective To explore the expression of miRNA-221 in human gliomas and its effect on glioma cell line proliferation.Methods The expression levels of miRNA-221 were determined by qRT-PCR in 123 samples of glioma tissues and 36 samples of normal cerebral tissues.The effects of miRNA-221 on U87 and U251 cell proliferation were determined by MIT tests.Dual luciferase assay,qRT-PCR and western blot were used to validate the targets of miRNA-221.Results The expression level of miRNA-221 (0.62+ 0.2) was significantly higher in the glioma tissues than that (0.37±0.13) in the normal cerebral tissues (P＜0.01).The survival time was significantly shorter in 72 glioma patients with higher expression of miRNA-221 than that in 51 glioma patients with lower expression of miRNA-221 after the operation (P＜0.01).The over expression of miRNA-221 significantly promoted U251 and U87 cells proliferations (P＜0.05).The depletion of miRNA-221 caused the increases in mRNA and protein of PRDM2 expressions (P＜0.05) in the U251 and U87 cells.Conclusions The upregulation of miRNA-221 may be used as one of diagnostic indexes of human glioma.PRDM2 is one of target genes of miRNA-221.
Keywords：Human gliomaMiRNA-221ProliferationExpressionPRDM2
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 463-466 )