Effect of radiotherapy combined with temozolomide on Ki-67 expression levels in primary cultured malignant glioma cells

WU Ke; TANG Shu-jun; Wei Jian-bo; HUANG Zhi-gang; WANG Dai-wen

doi:10.13798/j.issn.1009-153X.2017.05.013

Abstract：Objective To investigate the effect of postoperative radiotherapy and simultaneous temozolomide on the survival quality in the patients with gliomas and treatment of radiotherapy combined with temozolomide on Ki-67 expression levels in primary cultured malignant glioma cells.Methods One hundred and fourteen patients with malignant cerebral gliomas were randomly divided into two groups,i.e.control group (n=57),in which the patients received radiotherapy and observed group (n=57),in which the patients received chemotherapy with temozolomide on the basis of radiotherapy after the surgery for the gliomas.The gliomas tissues derived from the patients of each group were divided into two equal parts,of which,one as used for determination of Ki-67 expression and the other for the tissues culture.The cultured glioma tissues were derived into two groups,i.e.radiotherapy group,in which Ki-67 expression was determined in the cultured tissues after the radiotherapy and radiochemotherapy group in which Ki-67 expression was determined in the cultured tissues after the radiotherapy and chemotherapy with temozolomide.The survival quality was assessed by survival quality score before and after the treatment in all the patients.Results The survival quality scores [(43.40±3.26) points] were significantly higher in the observed group than that [(38.37±5.99) points] in control group after the treatment (P＜0.05).The positive rate of Ki-67 expression (24.6％) was significantly lower in the radiochemotherapy group than that (43.86％) in the radiotherapy group (P＜0.05).There was insignificant difference in the incidences of adverse reactions between the observed group and control groups (P＞0.05).Conclusions It is suggested that the radiotherapy combined with chemotherapy with temozolomide may increase the survival quality in the patients with malignant gliomas and decrease Ki-67 positive expression in the gliomas tissues.