Effect of ropivacaine discharged from nanoparticles in upper thoracic epidural space on delayed cerebral vasospasm in rabbits with SAH
SU Chang-sheng
WANG Li-ping
CHEN Guo-zhong
YANG Jian-teng
LIN Wen-qian
Abstract：Objective To investigate the effect of ropivacaine discharged from nanoparticles in the upper thoracic epidural on delayed cerebral vasospasm (DCVS) in rabbits after subarachnoid hemorrhage (SAH). Methods The upper thoracic epidural catheter were successfully placed in 75 healthy New Zealand rabbits which were randomly divided into five groups of 15 rabbits each, i.e. sham-operation group, control group, placebo nanoparticles group, ropivacaine-loaded nanoparticles group and pumping ropivacaine group. The models of SAH were established by two injections of autoblood into the cistern magnas. Neurobehavioral score was determined after SAH. Transcranial Doppler ultrasonography was used to detect the basilar artery (BA) mean flow velocity (Vm) and peak systolic blood flow velocity (Vp). The serum levels of neuron specific enolase (NSE) and S100B protein were measured by ELISA method. Morphological changes were observed under light microscope and tube diameters of BA were measured. The expressions of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) were measured by immunohistochemical technique in the endothelial cells of BA. Results The neurobehavioral score, degree of BA stenosis, Vm and Vp, level of ET-1 expression in the endothelial cells of BA and serum levels of NSE and S100B protein were significantly lower and the level of eNOS expression was significantly higher in ropivacaine-loaded nanoparticles group than placebo nanoparticles and control groups (P<0.01). Conclusion DCVS after SAH can be alleviated by ropivacaine discharged from nanoparticles in upper thoracic epidural space in the rabbits.
Keywords：Subarachnoid hemorrhageDelayed cerebral vasospasmUpper thoracic epidural spaceRopivacaineNanoparticles
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 540-544 )