Role of SNAI2 and E-cadherin in regulation of invasiveness and metastasis of human gliomas U251 cells by LRIG1

TAO Xiang; CHEN Chen; TAO Yun; ZHANG Shen-qi; LIU Bao-hui; LIAO Jian-ming; ZHANG Wen-fei; CHEN Qian-xue

doi:10.13798/j.issn.1009-153X.2016.09.009

Abstract：Objective To investigate the role of leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) in regulation of invasiveness and metastasis of human glioma U251 cells and the effects of SNAI2 and E-cadherin on it. Methods The cultured human glioma U251 cells were randomly divided into five groups, i.e. blank control group, LRIG1 vector group, LRIG1 over-expression group, LRIG1 low-expression group and LRIG1 low-expression negtive control group, in which the PBS, PEGFP-N1 plasmid, PEGFP-LRIG1 plasmid, LRIG1-siRNA and LRIG1-NC-siRNA wrer transfecte into the U251 cells, respectively. The mRNA and protein expressions of LRIG1, SNAI2 and E-cadherin were detected by real time-PCR and Western blotting, respectively. The invasiveness of U251 cells was determined by transwell chamber assay, and the migration of U251 cells was detected by transwell chamber assay with a matrigel coating and wound healing test. Results The levels of LRIG1 and E-Cadherin mRNA and proteins expressions were significantly higher in LRIG1 over-expression group than other groups (P<0.01), and they were significantly lower in LRIG1 low-expression group than blank control, LRIG1 vector group and low-expression negtive control groups (P<0.05). The levels of SNAI2 mRNA and protein expressions were significantly higher in LRIG1 low-expression group than other groups (P<0.05), and they were significantly lower in LRIG1 over-expression group than blank control, LRIG1 vector group and low-expression negtive control groups (P<0.01). The invasive and migration activities of U251 were significantly inhibited in LRIG1 over-expression group compared to other groups (P<0.01), and they were significantly enhanced in LRIG1 low-expression group compared to blank control, LRIG1 vector group and low-expression negtive control groups (P<0.01). Conclusion It is suggested that LRIG1 regulates the invasion and migration of gliomas U251 cells probably via the activation of SNAI2 and E-cadherin signaling pathway.