Resveratrol reduces cerebral ischemia-reperfusion injury through the activation of PI3K/Akt signaling pathway in rats
LEI Jun-rong
TU Xian-kun
ZHANG Hua-bin
ZHANG Jing
QIN Jun
LUO Jie
SHI Song-sheng
YANG Wei-zhong
Abstract：Objective To study the effect of resveratrol on cerebral injury induced by ischemic-reperfusion and its mechanism in rats. Methods Forty-eight SD rats were randomly divided into 4 groups of 12 animals each, including sham operation group, control group, experimental groupⅠin which the animal received the intraperitoneal injection of resveratrol 2 hours after the cerebral ischemia, and experimental groupⅡin which the animal received the intraventricular injection of LY294002 before the cerebral ischemia and the intraperitoneal injection of resveratrol 2 hours after the cerebral ischemia- reperfusion. The ischemia-reperfusion models were made by the suture occlusion in the control group, and experimental groupsⅠandⅡ. Neurological deficit scores, cerebral infarct volume and myeloperoxidase (MPO) activity in injured cerebral tissues were assessed 24 hours after cerebral ischemia-reperfusion. The expressions of p-Akt and t-Akt and content of tumor necrosis factor-α(TNF-α) in the injured cerebral tissues were detected by western blot and ELISA respectively in all the groups. Results The neurological deficit scores and cerebral infarct volume were significantly bigger in the control group 24 hours after the ischemia-reperfusion than those in the experimental groupⅠ(P<0.05), which were significantly smaller in the experimental group Ⅱ. The level of MPO activity and TNF-α in the injured cerebral tissues were significantly higher in the control group than those in the experimental groupⅡ(P<0.05), which were significantly higher in experimental groupⅠ(P<0.05), which were significantly higher in sham operation group (P<0.05). The levels of p-Akt and t-Akt expressions in the injured cerebral tissues were significantly higher in experimental group Ⅰthan those in experimental group Ⅱ(P<0.05), which were significantly higher in control group (P<0.05). Conclusions It is suggested that resveratrol can reduce cerebral ischemic-reperfusion injury and inflammatory reaction through the activation of PI3K/Akt signaling pathway in the rats.
Keywords：Cerebral ischemia-reperfusionResveratrolInflammatory reactionPI3K/AktRats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 425-428 )