Construction of siRNA recombinant plasmid targeting focal adhesion kinase gene and its effect on the proliferation and invasiveness of human glioma U251cells
HUANG Shu-lan
CHENG Yu-si
WANG Hui
WANG Chao-jia
Abstract：Objective To explore the effect of silencing focal adhesion kinase (FAK) gene on the proliferation and invasiveness of human glioma U251 cells. Methods U251 cells were divided into 3 groups, i.e. blank group, negative control group and experiment group. The FAK small interference RNA (siRNA) recombinant plasmid was transfected into human glioma U251 cells in the experimental group. The expressions of FAK mRNA and protein were detected by real time PCR and Western-blot respectively, and the U251 cells proliferative and invasive capabilities were measured respectively by RT cell analyzer (RTCA) and Transwell chamber invasion assay. Results The pBSilence1.1-FAK recombinant plasmid was successfully constructed. RTCA results showed that U251 cell proliferative capability was significantly lower in the experimental group than those in the blank group and the negative control group (P<0.05). Transwell chamber invasion assay showed that the number of U251 cells of transwell chamber [(17.137 ± 1.012) cells] was significantly fewer in the experimental group than those in the blank group [(68.218 ± 4.547) cells] and the negative control group [(67.034±2.272) cells] (P<0.05). Conclusion Silencing FAK gene can significantly inhibit proliferation and invasveness of human glioma U251 cells in vitro.
Keywords：GliomaU251 cellsFocal adhesion kinaseSmall interference RNAProliferationInvasiveness
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 336-340 )