Effect of methylation levels of GDNF gene promoterⅠregion on its transcription in glioma U251 cells
XU Ji-jun
MENG Zhu
LI Zhi-peng
CHEN Xin
REN Qing-xian
QIN Han
Abstract：Objective To explore the effect of methylation levels of glia cell line-derived neurotroplic factor (GDNF) promoterⅠregion on its transcription in glioma U251 cells. Methods The methylation levels of GDNF promoterⅠregion were detected before and after intervention with different concentrations of 5-aza-CR in glioma U251 cells. The GDNF mRNA expression was detected by RT-PCR in each group. Results The methylation levels of GDNF gene promoterⅠregion declined after the intervention with 5-aza-cR compared to the control group. The methylation levels of GDNF gene promoterⅠregion were significantly lower in 5μmol, 10μmol and 20μmol 5-aza-CR intervention groups than that in the control group (P<0.05). The level of GDNF mRNA expression in U251 cells was significantly higher in the 5 μmol 5-aza-CR intervention group than those in the control group and 1 μmol, 10 μmol and 20 μmol intervention groups (P<0.05). There were insignificant differences in the levels of GDNF mRNA expression in U251 cells among the control group and 1μmol, 10μmol and 20μmol 5-aza-CR intervention groups (P>0.05). Conclusion The demethylation of GDNF gene promoterⅠregion, which is produced by 5-aza-CR, may influence the expression of GDNF in U251 cell.
Keywords：GliomaGDNFU251 cellsPromoterMethylationExpression
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 410-412 )