Effect of RNA interference on expression of c-Met gene in U251 cells
WANG Chao-jia
WANG Hui
HU Jun-tao
Abstract：Objective To construct the shRNA expression vector which targeted c-Met gene and explore the effect of the vector on the expression levels of c-Met gene in the U251 cells. Methods Three pairs of pGenesil-1-c-Met-shRNA recombinant plasmids were constructed. The recombinant plasmids were transfected into the U251 cells. The c-Met mRNA and protein expression levels were determined respectively by the RT-PCR and Western blot methods. Results The pGenesil-1-c-Met shRNA recombinant plasmids were successfully constructed and transfected into U251 cells. The levels of c-Met mRNA expression and protein expression were significantly lower in the U251 cells transfected with pGengsil-1-c Met shRNA recombinant plasmids than those in the U251 cells untransfected with the recombinant plasmids (P<0.05). Conclusion RNA interference can significantly reduce c-Met mRNA and protein expression level in the U251 cells.
Keywords：U251 cellsC-Met genesTransfectionRNA interference
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 99-100 )