Ginsenoside Rd pretreatment attenuates glutamate-induced injury of PC12 cells
YANG Jun-zhe
ZHOU Xiang
CHENG Peng-fei
SONG Xiao-yang
LUO Zhong-bing
ZHOU Jing-song
TAO Jun
Abstract：Objective To investigate the effect of ginsenoside Rd on the glutamate(Glu)-induced injury of PC12 cells. Methods PC12 cells were invided into three groups, i.e. control group, Glu group and ginsenoside Rd pretreatment group. PC12 cells in the control group were cultured in Glu-free DMEM medium. PC12 cells in Glu group were exposed in the DMEM medium containing 10 mmol/L Glu for 24 h. PC12 cells in ginsenoside Rd pretreatment group were pretreated with 50μmol/L ginsenoside Rd for 30 minutes, and then were cultured in DMEM medium containing to 10 mM Glu for 24 h. MTT method was used to determine the cell survival. Lactic dehydrogenase (LDH) level was assessed in the medium to analyze the injury level of the cells. The reactive oxygen species (ROS) level in the medium was determined by flow cytometry. SOD reagent kit was used to determine the intracellular SOD level in the medium. The morphology of PC12 cells was observed by the phase contrast microscope. Results The LDH and ROS levels in the medium which were (857.4 ± 93.6)U/L and (190.2 ± 24.1)%respectively were significantly lower in the ginsenoside Rd pretreatment group than those, which were (1461.0 ± 138.1)U/L and (354.7 ± 48.1)% respectively, in Glu group (P<0.05). The SOD level in the medium [(69.5 ± 11.2)%] was significantly higher in ginsesnoside Rd pretreatment group than that [(41.9±6.3)%] in Glu group (P<0.05). The cellular morphology was significantly ameliorated in ginsesnoside Rd pretreatment group compared to Glu group. Conclusion Ginsenoside Rd pretreatment can ameliorate PC12 cells injured by Glu.
Keywords：Ginsenoside RdPretreatmentGlutamateOxidative stressPC12 cells
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 543-545 )