Effects of Electroacupuncture on Learning and Memory and Expression of Brain-derived Neurotrophic Factor and p75 Neurotrophin Receptor in Rats with Cerebral Ischemia-reperfusion
LI Xiao-jie
LIN Ru-hui
TAO Jing
ZHAO Cong-kuai
YU Kun-qiang
LI Chun-yan
PENG Hong-wei
CHEN Li-dian
Abstract：Objective To observe the effects of electroacupuncture on learning and memory, and discuss the therapeutic mechanism. Methods 45 Sprague-Dawley rats were randomly divided into sham group (n=15), model group (n=15) and electroacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 1.5 h and reperfusion. The rats of electroacupuncture group received electroacupuncture at Shenting (DU24) and Baihui (DU20) for 7 days. Learning and memory ability was tested with Morris water maze. Neurologic impairment was assessed with Longa's score. Their hippocampus were observed under HE staining and the level of brain-derived neurotrophic factor (BDNF) and p75 neurotrophin receptor (p75NTR) protein were determined with Western blotting. Results Compared with the model group, the latency of water maze decreased and the times crossing the platform increased in electroacupuncture group (P<0.05), while the Longa's score significantly reduced (P<0.05), and the lesion of nerve cells were alleviated, with the decrease of p75NTR and increase of BDNF in the ischemic hippocampus (P<0.01). Conclusion Electroacupuncture can improve the learning and mem-ory of cerebral ischemia-reperfusion rats, which may relate with up-regulating BDNF and down-regulating p75NTR in hippocampus.
Keywords：cerebral ischemia-reperfusionlearning and memoryelectroacupuncturebrain-derived neurotrophic factorp75 neuro-trophin receptorrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1020-1024 )