Effect of Butylphthalide on Apoptosis of SH-SY5Y Cells
ZHAO Su-chen
WU Qing-wen
CHENG Yue-fa
CHEN Juan
JIA Yu-feng
MENG Qi
Abstract：Objective To explore the effects of butylphthalide on the apoptosis of SH-SY5Y cells induced by the 1-methyl-4-phenylpyri-dinium iodide (MPP+). Methods SH-SY5Y cells were cultured with butylphthalide in the dosage of 1 μmol/L, 10 μmol/L and 20 μmol/L for 2 hours, and with MPP+for 24 hours. The viability of cells was measured with trypan blue. The mitochondrial membrane potential was de-tected with Rhodamine 123. The content of P53 protein was detected with ELISA. The expression of P53 and cytochrome C (CytC) were de-tected with Western blotting. Results The viability of SH-SY5Y cells increased with the dosage of butylphthalide (P<0.05), while the mito-chondrial membrane potential and the expression of P53 and CytC decreased (P<0.05). Conclusion Butylphthalide can prevents the SH-SY5Y cells from the injury induced by MPP+, which may associate with the inhibition of apoptosis through the expression of P53 and CytC.
Keywords：Parkinson's diseasebutylphthalideSH-SY5Y cellsP53cytochrome Capoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 412-416 )