Basic Fibroblast Growth Factor-Chitosan Carriers Induce Neural Stem Cells to Differentiate into Neurons and Form Synapses
WANG Cong
YANG Zhao-yang
DUAN Hong-mei
LI Xiao-guang
Abstract：Objective To explore the effect of basic fibroblast growth factor (bFGF)-chitosan carriers on neural differentiation of neural stem cells (NSCs). Methods NSCs were isolated from spinal cord of a neonatal Wistar rat and cultured. Purity of cultured NSCs was identi-fied with Nestin immunofluorescent staining. The 10 mg/ml chitosan carriers, 20 ng/ml bFGF or 10 mg/ml bFGF-chitosan carriers were add-ed into medium of P3~P4 NSCs respectively. NSCs were observed with immunofluorescent staining: 3 days after incubation with Nestin andβ-tubulin III;7 days after incubation with microtubule-associated protein-2 (MAP2), glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP);and 14 days after incubation with synapsin-1 and MAP2. The electrophysiological activity of cells was detected with MED64. Results 3 days after incubation, all the NSCs differentiated into Nestin+/β-tubulin III+, and the length of neurofilament was the high-est in those co-cultured with bFGF-chitosan carriers. 7 days after incubation, NSCs differentiated into MAP2+, GFAP+and MBP+, and more NSCs differentiated into MAP2+with bFGF-chitosan carriers. 14 days after incubation, NSCs differentiated with bFGF-chitosan carriers ex-press synapsin-1+/MAP2+and showed electrophysiological activity. Conclusion bFGF-chitosan carriers can induce NSCs to differentiate in-to neuron with high percentage and the differentiated neurons can form synapses with electrophysiology activity.
Keywords：neural stem cellsbasic fibroblast growth factorchitosanneural differentiationsynapsesneurons
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 406-411 )