Effects of Heat Preconditioning on Expression of Protein Kinase C in Myocardium of Rats after Overload Exercise
YANG Yi
LI Zhang-hua
SHI Wu-di
LIU Hua
ZHANG Jie
Abstract：Objective To investigate the effects of heat preconditioning (HP) on the expression of protein kinase C (PKC)δand PKCεin the myocardium of overload exercise rats. Methods 25 male three-month-old Sprague-Dauley rats were divided into control group (n=5), exercise group (n=10) and HP group (n=10). The expression of PKCδand PKCεin the myocardium was detected with immunohistochemis-try, real-time PCR and Western blotting 8 weeks after overload exercise. Results The expression of PKCδsignificantly increased in the exer-cise group compared with the control group and the HP group (P<0.05). In another hand, PCR and immunohistochemistry showed the ex-pression of PKCεin the exercise group decreased compared with the control group and the HP group (P<0.05), but it was not significantly different among the groups using Western blotting (P>0.05). Conclusion PKCs may play an important role in the HP-induced cardio-protec-tion during overload exercise.
Keywords：heat preconditioningmyocardiumprotein kinase Coverload exerciserats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 49-53 )