Effect of Electroacupuncture on Learning and Memory Ability of Cerebral Ischemia/Reperfusion Injured Rats
LIN Yu-kun
TAO Jing
CHEN Bin
LIN Ru-hui
Abstract：Objective To investigate the effect of electroacupuncture at Shenting (DU24) and Baihui (DU20) on learning and memory ability, as well as the nerve cell and the expression of mRNA of Bcl-2 and Bax in the hippocampus of rats with cerebral ischemia/reperfu-sion (I/R) injury. Methods 45 male Sprague-Dawley rats were randomly diveded into sham group (n=15), control group (n=15) and elec-troacupuncture group (n=15). The latter 2 groups were modeled with middle cerebral artery occlusion for 2 h and reperfusion. The elec-troacupuncture group received electroacupuncture at Shenting and Baihui for 7 days. They were tested with Morris water maze, and then, their hippocampus was observed under Nissl staining and the level of Bcl-2 and Bax mRNA were determined with RT-PCR. Results The es-cape latency decreased (P<0.001) and the frequence the platform was crossed increased (P=0.001) in the electroacupuncture group com-pared with the model group, with less damage of the nerve cell and the expression of Bax mRNA, and more of Bcl-2 mRNA (P<0.05). Con-clusion Electroacupuncture can prevent learning and memory ability from cerebral ischemia/reperfusion injury in rats, which may associate with adjustment of expression of apoptosis-related gene to inhibit apoptosis of nerve cell.
Keywords：cerebral ischemia/reperfusionelectroacupuncturelearning and memoryapoptosisBaxBcl-2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 831-834 )