Effects of Cluster Scalp Acupuncture Combined with Enriched Environment Stimulation on Proliferation and Migration of Endoge-nous Neural Stem Cells in Neonatal Rats with Hypoxic-ischemic Brain Damage
Abstract：Objective To explore the effect of cluster scalp acupuncture combined with enriched environmental stimulation on prolifera-tion and migration of endogenous neural stem cells (NSCs) in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods HIBD model was established with Rice method. 120 rats were randomly divided into sham operation group (A), model group (B), cluster scalp acu-puncture group (C), enriched environmental stimulation group (D) and cluster scalp acupuncture combined with enriched environmental stimulation group (E) with 24 rats in each group, each group was divided into 3 days, 7 days and 14 days subgroups. Immunohistochemical method was used to observe the positive cells of Bromodeoxyuridine (BrdU) and nestin. Results Compared with Group B, the positive cells number of BrdU and nestin in subventricular zone (SVZ) and cerebral cortex increased in Groups C and D at all the time points (P<0.01), and the number was more in Group E than in Groups C and D (P<0.05). Conclusion Cluster scalp acupuncture combined with enriched en-vironmental stimulation could promote the proliferation and migration of endogenous NSCs in SVZ and cerebral cortex in neonatal rats with HIBD.
Keywords：hypoxic-ischemic brain damagecluster scalp acupunctureenriched environmental stimulationproliferationmigrationrats
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 533-536 )