Establishment of Animal Model of Early Osteonecrosisof the Femoral Head with Modified-induced Steroid Method
Abstract：Objective To explore the method to establish animal model of early osteonecrosis of the femoral head (ONFH) induced by steroid. Methods 20 healthy male Beagles were randomly divided into control group and experimental group with 10 dogs in each group. The experimental group was injected lipopolysaccharide 10μg/kg and methylprednisolone 20 mg/kg for 3 days consecutively. The control group was injected normal saline. 2 months and 4 months after administration, both groups were performed magnetic resonance imaging (MRI). 5 animals were sacrificed respectively at 2 months and 4 months after administration in each group, and bilateral femoral head speci-mens were obtained to perform histological examination. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), anti-thrombin III (AT-III) were tested before and 24 h after administration. Results In the experimental group, the pathological results showed that there were 4 ONFH 2 months and 6 ONFH 4 months after administration and MRI did not show any abnormality. Compared with the control group, the PT, APTT, AT-III in the experiment group shortened significantly after administration (P<0.001). Conclusion Modified steroid-induced method can establish the animal model of early ONFH. Hypercoagulation and low fibrinolysis may be the reason of ste-roid-induced osteonecrosis.
Keywords：osteonecrosis of the femoral headearly stagemodified steroid-induced methodanimal modelBeagles
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 527-532 )