Effect of Celecoxib on Proliferation and Apoptosis of U251 Glioma Cells
Abstract： Objective To explore the effect of celecoxib on proliferation and apoptosis of the glioma cells. Methods The glioma cell U251 was used and disposed with different densities of celecoxib (0μmol/L, 10μmol/L, 20μmol/L, 40μmol/L and 80μmol/L) for 24 h, 48 h and 72 h. The methyl thiazolyl tetrazolium (MTT) assay was used to detect the tumor cell proliferation and flow cytometry was used to de-tect the tumor cell apoptosis rate. Results The Glioma U251 cells proliferation were significantly decreased with the increase of density of celecoxib in vitro, and there was significant difference in the inhibited rate in different density and different time (P<0.05). The apoptosis rate was higher in the density of 80μmol/L (17.86%) than in that of 0μmol/L (11.23%) (P<0.05). Conclusion Celecoxib can inhabit the pro-liferation of glioma U251 cells, and promote the apoptosis especially with the density of 80μmol/L.
Keywords：gliomaU251 cellcelecoxibproliferationapoptosis
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 532-535 )