The effect and mechanism of sulforaphane on alleviating cerebral injury in pigs after resuscitation
Zhou Meiya
Xu Jiefeng
Cao Guangli
Wang Xuguang
Ge Feng
Zhu Jinjiang
Wang Jiangang
Zhang Mao
Abstract：Objective To investigate the effect and mechanism of sulforaphane(SFN)in reducing cerebral injury and neurological dysfunction after cardiopulmonary resuscitation(CPR).Methods A porcine model of cardiac arrest(CA)and CPR in pigs(CA 10 min+CPR 6 min)was established.Animals were randomly divided into three groups:Sham group(n=6),CPR group(n=9),and CPR+SFN group(n=9).In the CPR+SFN group,we administered 2 mg/kg SFN dissolved in 20 mL saline through the femoral vein at 5 minutes after successful modeling.The administration was completed within 10 minutes.Serum levels of brain injury markers(NSE and S100β)were measured at 1 hour,2 hours,4 hours,and 24 hours after resuscitation.At 24 hours post-resuscitation,pathological brain damage,levels of proinflammatory factors,and expression of proteins related to the nuclear factor erythroid 2-related factor-2(Nrf2),heme oxygenase 1(HO-1),and nuclear factor-κB(NF-κB)pathways were assessed.Results ①Baseline indexes such as body weight,HR,MAP,CBF,ETCO2,pH and lactic acid before modeling(all P＞0.05).②During resuscitation,the parameters of CPR cycle,adrenalin dosage and defibrillation times were comparable between the CPR group and the CPR+SFN group(all P＞0.05).The resuscitation success rates were 6/9 and 7/9,respectively(P＞0.05).③Compared with the CPR group,the CPR+SFN group showed increased MAP at 2 h,3 h and 4 h,with a significant difference at 3 h(P＜0.05).④Serum NSE and S100β levels in the CPR+SFN group at 2 h,4 h and 24 h were lower than those in the CPR group(P＜0.05).⑤The pathological changes of brain tissue in the CPR+SFN group were milder than those in the CPR group.⑥The Iba1 positive expression ratio,TNF-α and IL-6 contents in CPR+SFN group were significantly lower than those in CPR group(all P＜0.05).⑦Compared with the CPR group,the protein expression levels of Nrf2 and HO-1 in the CPR+SFN group were further significantly increased,while the protein expression level of NF-κB was significantly decreased in CPR+SFN group(all P＜0.05).Conclusions SFN alleviates post-resuscitation brain injury in a porcine model,and the mechanism may be associated with the attenuation of inflammatory injury via modulation of the Nrf2/HO-1/NF-κB pathway.
Keywords：SulforaphaneCardiac arrestCardiopulmonary resuscitationNrf2/HO-1/NF-κB pathwayCerebral injuryInflammationPig
Publication Date：2026-01-10
Online Publishing Date：2026-01-29(First online date of this platform, not the publication date of the document)
Pages：7( 14-20 )