Study on the mechanism of using Tiaochang formula enema to improve acute lung injury in sepsis based on regulating inflammatory factors

Zhang Ruifen; Su He; Jia Tingting; Zhang Hairong; Li Yang; Lyu Xinyue; Rong Jilintai; Wang Hao; Li Yan

doi:10.3969/j.issn.1002-1949.2025.08.008

Abstract：Objective To observe the effect of the Tiaochang formula(TCF)enema treatment on the level of inflammatory factors in the rats with sepsis-associated acute lung injury,and to explore the mechanism of action.Methods A total of 60 male SD rats were randomly allocated into the sham group,model group,low-dose TCF group,medium-dose TCF group,and high-dose TCF group,with 12 rats in each group.The sepsis-associated acute lung injury model was established by cecal ligation and puncture(CLP).The sham group and the model group were administered normal saline,while the low-dose,medium-dose,and high-dose TCF groups were given 2.2,4.4,and 8.8 g/d TCF liquid per day respectively.The status of the rats was observed three days after administration.Behavioral score,arterial blood gas,the levels of interleukin-6(IL-6)and interleukin-10(IL-10)in serum and lung tissue were measured.The intestine and left lung tissues after hematoxylin-eosin(HE)staining were used to observe the degree of tissue damage.The right lung tissue was dried,and the lung wet-to-dry weight ratio(W/D)was compared.Results Compared with the sham group,the behavioral scores of rats in the other groups increased,the level in the model group was notably higher than that in the other groups(P＜0.05).Compared with the sham operation group,the PaO2 level and PaO2/FiO2 index in the model group were significantly decreased,while the PaCO2 level was significantly increased(P＜0.05);Compared with the model group,the PaO2 level and PaO2/FiO2 index in the TCF group were significantly increased,and the PaCO2 level was significantly decreased(P＜0.05),with the high-dose group showing better improvement than the low-dose and medium-dose groups.Compared with the sham operation group,the W/D ratio of lung in the model group increased significantly(P＜0.05);Compared with the model group,the W/D ratio in the TCF group decreased significantly(P＜0.05),and the high-dose group showed a lower ratio than the low-dose and medium-dose groups.Compared with the sham group,the model group showed severe structural damage in the lungs and intestines,massive infiltration of inflammatory cells,and capillary dilation with hemorrhage.In the low-dose,medium-dose,and high-dose TCF groups,the tissue damage in the lungs and intestines of rats was alleviated,with reduced structural destruction.Compared with the sham group,the IL-10 levels were significantly lower in the remaining group,and IL-10 levels in the model group was lower than that in TCF group(P＜0.05);compared with the model group,the IL-6 levels in the TCF group was significantly lower(P＜0.05).Conclusions TCF can effectively reduce the degree of lung structural damage caused by sepsis in rats and alleviate the level of inflammatory response.