The role of absent in melanoma 2 inflammasome-mediated pyroptosis in hypothalamic injury caused by cold-water immersion hypothermia in rats
Dai Jing
Yu Xinhui
Wang Zewei
Cheng Feng
Liu Ying
Jin Hongxu
Abstract：Objective To investigate the role of absent in melanoma 2(AIM2)inflammasome-mediated pyroptosis in hypothalamic injury induced by cold-water immersion hypothermia in rats.Methods Twenty rats were randomly divided into control group(n=10)and model group(n=10).The model group accepted cold-water immersion to establish rat models of hypothermia.During the process,the heart rate was monitored to evaluate the hypothalamic blood supply.The degree of hypothalamic nerve cell damage was evaluated by HE staining,nerve injury score and Nissl staining method.The expressions of AIM2,apoptosis-associated speck-like protein containing CARD(ASC),cysteinyl aspartate specific proteinase-1(Caspase-1),interleukin(IL)-1β,IL-18,gasdermin D(GSDMD),S100 calcium-binding protein beta(S100β)and IL-6R in hypothalamus were determined by Western blot and immunofluorescence staining method.Results The changes of heart rate and core temperature were consistent in model group.Compared with the control group,the hypothalamic nerve injury score increased in the model group(P＜0.05),the expressions of S100β in hypothalamus were higher(P＜0.05),and the pathological injury degree of brain tissue aggravated significantly.The expressions of IL-1β,IL-18,IL-6R in hypothalamus increased(P＜0.05),and the expressions of pyroptosis-related proteins such as AIM2,ASC,Caspase-1,GSDMD increased in model group(P＜0.05).Conclusions Cold-water immersion hypothermia can induce hypothalamic injury,which may be related to the classical pyroptosis pathway mediated by AIM2.
Keywords：Absent in melanoma 2PyroptosisHypothalamic injuryHypothermia
Publication Date：2025-03-09
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 192-198 )