The value of the 5'tRF-LysCTT content in the blood for the early diagnosis and evaluation of disease severity in the patients with acute cerebral infarction
Su Qian
Meng Delong
Wang Mengke
Su Zhiqiang
Li Guozhong
Abstract：Objective To explore the value of the 5'tRF-LysCTT expression level in the blood for the early diagnosis and evaluation of disease severity in the patients with acute cerebral infarction(ACI).Methods Clinical data of hospitalized ACI patients in the Neurology Department of the First Affiliated Hospital of Harbin Medical University from October 2022 to October 2023 were collected.A total of 114 ACI patients were selected as ACI group,and 112 healthy patients in the same period were selected as the control group.ACI patients were classified into mild group(NIHSS≤5 scores,n=66)and moderate to severe group(NIHSS＞5 scores,n=48)based on the national institutes of health stroke scale(NIHSS).The 5'tRF-LysCTT levels in both groups were measured by quantitative real-time polymerase chain reaction(RT-qPCR)technology.Multivariate Logistic regression analysis was applied to predict risk factors for the degree of neurological deficit in ACI patients.ROC curves were used to analyze the diagnostic value of 5'tRF-LysCTT for distinguishing mild ACI from moderate to severe ACI.Results The peripheral blood 5'tRF-LysCTT levels were significantly lower in the ACI group compared with the control group(1.34±0.56 vs.3.28±1.01,P＜0.001).The 5'tRF-LysCTT expression in the moderate to severe group was significantly reduced compared with the mild group(0.89±0.36 vs.1.65±0.45,P＜0.001).The results of ROC curve analysis showed the AUC value of 5'tRF-LysCTT for distinguishing the ACI group from the control group was 0.956(95％CI 0.9317-0.9805),with a sensitivity of 94.6％,the specificity of 84.7％,the cutoff value of 2.11.At the same time,the AUC value of 5'tRF-LysCTT for distinguishing between mild ACI and moderate to severe ACI was 0.908(95％CI0.8543-0.9618),with a sensitivity of 93.3％,the specificity of 80.2％,the cutoff value of 1.41,the diagnostic efficiency was high.Logistic regression analysis found 5'tRF-LysCTT was an independent risk factor for predicting the degree of neurological deficit.Conclusions The 5'tRF-LysCTT in the blood of ACI patients may be a new marker for early diagnosis and determination of disease severity.
Keywords：Acute cerebral infarctiontRNA-derived fragments5'tRF-LysCTTMarkerEarly diagnosis
Publication Date：2024-08-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 733-737 )