Study of small non-coding RNA changes in brain tissue of exertional heat stroke mice based on Pandora sequencing
Liu Ying
Shi Xiuyun
Hu An
Liu Chunyang
Dai Jing
Wang Zewei
Cheng Feng
Cao Binyan
You Pingfei
Jin Hongxu
Abstract：Objective To explore the changes of small non-coding RNAs(sncRNAs)in brain tissue of exertional heat stroke(EHS)mice based on Pandora sequencing.Methods Twelve healthy male C57BL/6J mice were randomly divided into control group(n=6)and model group(n=6).The mice in the control group were not treated,and the mice in the model group ran in a constant temperature and humidity box to establish a EHS model.After the EHS model was established,the brain injury of mice was determined based on wet and dry weight detection and HE staining.Differential sncRNAs were screened by Pandora sequencing.Results A total of 2 722 sncRNAs were detected by PANDORA-Seq.Compared with normal control group,fold change＞2 and P＜0.05 were selected as screening conditions,53 sncRNAs with significant differences were identified in brain tissues of mice with EHS,such as mmu-miR-1843b-5p,mmu-miR-490-3p,mmu-miR-1843a-5p,piR-mmu-7796454 and tsRNA-1020-MetCAT.Based on the small RNA annotation software SPORTS1.1 for the discovery and annotation of sncRNAs,the length distribution map and species distribution radar map were provided.The difference analysis results of sncRNA species and sequence were shown by heat map and volcano map.Conclusions Dysregulation of sncRNAs plays a role in the pathogenesis of brain injury in EHS mice.
Keywords：Heat strokeBrain injuryPandora sequencingSmall non-coding RNAs
Publication Date：2024-06-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 503-508 )