The changes of plasma protein in the rats with exertional heat stroke by DIA proteomics

Lin Qingwei; Luo Zhuqing; He Longping; Yu Yongchun; Zeng Junjie; Song Jingchun

doi:10.3969/j.issn.1002-1949.2024.06.005

Abstract：Objective To analyze the changes of plasma protein expression in the rats with exertional heat stroke(EHS)by using data-independent acquisition(DIA)proteomics.Methods Ten specific pathogen-free(SPF)male SD rats were randomly divided into a normal control group(n=5)and an EHS group(n=5).The EHS group underwent temperature telemetry capsule implantation surgery 1 week before modeling,and after one week of recovery,the rats ran in an artificial climate chamber(temperature 40 ℃,relative humidity 70％)until the core temperature reached 42.5 ℃,which was considered the occurrence of EHS.Blood was collected after running to analyze the DIA quantitative proteomics.Results A total of 625 upregulated differentially expressed proteins(DEPs)and 8 downregulated DEPs were screened by using the criteria of expression multiple＞1.5 times and P＜0.05.Subcellular localization annotation showed that DEPs were mainly located in the extracellular space and cytoplasm.Gene ontology(GO)annotation showed that DEPs mainly functioned as protein binding.Kyoto encyclopedia of genes and genomes(KEGG)annotation showed that DEPs mainly participated in immune response,energy metabolism,and other signaling pathways.Clusters of orthologous groups of proteins(COG)annotation showed that DEPs mainly played roles in post-translational modification,protein turnover and chaperone function.Protein domain enrichment analysis showed that the protein domains of DEPs were mainly concentrated in immunoglobulin domains,platelet reactivity protein 1 type domains,and complement Clr-like EGF-like domains.GO enrichment analysis showed that DEPs mainly participated in blood coagulation and fibrin clot formation,fibrinolytic enzyme activation,and acute inflammatory response regulation.KEGG enrichment analysis showed that DEPs mainly participated in the complement and coagulation cascades,immune and cell adhesion molecule signaling pathways.Reactome pathway enrichment analysis showed that DEPs mainly participated in the innate immune system,platelet activation,and neutrophil degranulation signaling pathways.Protein interaction network analysis found that the protein interaction network of the complement and coagulation cascades was more interactive,and the upregulated proteins Fgg,Plg and Serpinc1 were the main ones.Conclusions EHS significantly changes the protein expression profile in rat plasma,mainly involving the pathways of abnormal activation of the immune system,out-of-control inflammatory response,and coagulation disorder.