Distribution characteristics of T lymphocytes in the serum and lungs of sepsis rats
Zhao Jiwei
Chen Tonghua
Mai Haochen
Chen Xueru
Zhang Meng
Abstract：Objective To study the distribution characteristics of T lymphocytes in the serum and lungs of sepsis rats.Methods 20 SPF-grade SD rats were randomly divided into Sham 24 h,Sham 48 h,cecal ligation and puncture(CLP)24 h and CLP 48 h.Sepsis models were established by CLP.Meanwhile,peripheral blood and bronchoalveolar lavage fluid(BALF)were collected 24 h and 48 h after modeling,and flow detection of CD3+ and CD4+ T lymphocytes was performed.The pathological changes of lung tissue were observed by hematoxylin-eosin(HE)staining light microscope,and the pathology of lung injury was also scored.Results Compared with the corresponding time of Sham group,the proportions of serum CD3+ and CD4+ T lymphocytes in CLP group were decreased at 24 h and 48 h after CLP(all P<0.05).Serum CD3+ and CD4+T lymphocytes in Sham group and CLP group had different degrees of proliferation at 48 h after CLP compared with 24 h after CLP,but the degree of proliferation in CLP group was lower than that in Sham group(all P<0.05).The proportions of CD3+ and CD4+T lymphocytes in BALF in CLP 24 h group were lower than those in Sham 24 h group(all P<0.05).The proportions of CD3+ and CD4+ T lymphocytes in BALF in CLP 48 h group were significantly higher than those in CLP 24 h group(P<0.05).The rats in different groups had different degrees of lung injury,and the lung injury scores were higher in CLP 24 h group with a large number of inflammatory cell infiltration,pulmonary interstitial hyperemia and edema,and alveolar hemorrhage(P<0.05).Conclusions The proliferation of T lymphocytes in the serum and lung of sepsis rats is inhibited in the middle and late stages of sepsis.The levels of serum CD3+ and CD4+T lymphocytes can reflect the level of lung tissue to a certain extent.The sepsis-induced lung injury is the more severe,the proliferation of T lymphocytes in BALF tends to decrease.
Keywords：SepsisRatBronchoalveolar lavage fluid(BALF)T lymphocytesCD3+CD4+
Publication Date：2024-05-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 443-448 )