Effects of exogenous hydrogen sulfide on redox equilibrium of septic macrophages

Wang Xing-cheng; Yang Lei; Wang Bin - sheng; Peng Chao; Ma Li - bin; Chai Chen

doi:10.3969/j.issn.1002-1949.2019.01.016

Abstract：Objective To explore the effects of exogenous hydrogen sulfide ( H2S) on redox balance of macrophages in sepsis. Methods The macrophage strain J774 was divided into phosphate buffer saline (PBS) (1 mL/mL) control group, lipopolysaccharide (LPS) (1 μg/mL) group, DL-propargylglycine (PAG) (1 mmol/L) +LPS (1 μg/mL) group, GYY4137 (500 μmol/L) group and GYY4137 (500 μmol/L) +LPS (1 μg/mL) group. In the supernatant of cell culture of each group, the levels of reactive oxygen species ( ROS), malondialdehyde ( MDA), superoxide dismutase ( SOD), catalase ( CAT) and glutathione peroxidase ( GSH -Px) were measured by ELISA. The changes of mitochondrial morphology in cells were observed by electron microscope. Results Finally, the average concentration of ROS in supernatant of LPS group was (90. 293 ± 0. 217) U/mL, MDA was (1. 665 ± 0. 068) nmo/mL, SOD was (89. 857 ± 3. 002) U/mL, CAT was (8. 099 ± 0. 130) U/mL, GSH-Px was (10. 287 ± 0. 335) μmol/L. In the supernatant of GYY4137+LPS group, the average concentration of ROS was (25. 819 ± 2. 988) U/mL, MDA was (0. 355 ± 0. 065) nmol/mL, SOD was (189. 690 ± 0. 290) U/mL, CAT was (189. 690 ± 0. 290) U/mL, GSH-Px was (287. 235 ± 0. 864) μmol/L. The levels of ROS and MDA in the GYY4137 +LPS group were significantly lower than those in the LPS group with significant difference(P <0. 05). The levels of antioxidant SOD, CAT andGSH -Px were considerably higher with significant difference ( P <0. 05), and the degree of damage to cells and mitochondria was also lower. Conclusion Hydrogen sulfide has a protective effect on the redox balance of septic macrophages, which may be due to its direct inhibition of mitochondrial oxidative stress.