Anesthetic post-conditioning at the initiation of CPR attenuates cerebral injuries in a rat model of cardiac arrest
Li Heng-jie
Mao Hui
Cai Wen-wei
Yang Yan
Wei Hong-yan
Li Bo
Lu Yuan-zheng
Liao Xiao-xing
Abstract：Objective In this study,we hypothesized that anesthetic post-conditioning with sevoflurane at the initiation of cardio-pulmonary resuscitation (CPR) will attenuate cerebral injuries in the model of cardiac arrest.Methods The cardiac arrest model was established through asphyxia.Forty male Wistar rats were assigned to two groups randomly.Rats in the sevoflurane (anesthetic post-conditioning,APoC) group inhaled 1 MAC of sevoflurane during CPR.Rats in the control (CON) group did not receive inhaled sevoflurane during CPR.After return of spontaneous circulation (ROSC),the mean arterial pressure (MAP) and heart rate (HR) was recorded.Before cardiac arrest (baseline) as well as ROSC 1 h and ROSC 4 h,cardiac function was measured.Neurologic deficit scores (NDS) was assessed at 24 and 72 hours after ROSC.At 72 hours after ROSC,coronal brain sections were analyzed by counting TUNEL positive (i.e,apoptotic) cells and Nissl positive (i.e,viable) cells.Results There was no statistical difference in MAP and HR between the two groups.Rats in the APoC group had a thinner LVPW (P ＜ 0.05) and a higher EDV (P ＜ 0.01) compared to the CON group.The apoptotic cells were less and the viable neurons were more in the APoC group compared with CON group (P ＜ 0.05).The NDS was higher in the APoC group at ROSC 24 h and ROSC 72 h (P ＜ 0.05).Conclusion Volatile anesthetic sevoflurane,when administered at initiation of CPR,improves NDS associated with reducing neuronal damage.
Keywords：SevofluraneAnesthetic post-conditioningCardiac arrest (CA)Cardio-pulmonary resuscitation (CPR)Brain injuries
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 420-424,后插2 )