MicroRNA-222 modulates the migratory ability of endothelial progenitor cells from human peripheral blood through the Akt signaling pathway
Xu Wen-feng
Wang Cui-ping
Zhang Hao
Cai Hua-zhong
Shi Liang
Sun Wen-wen
Ren Guo-qing
Abstract：Objective To explore the effects of microRNA-222 (miR-222) on the migration of endothelial progenitor cells (EPCs) and the role of Akt signaling pathway and its target gene, ETS-1(ETS proto-oncogene 1,transcription factor), in this process. Methods EPCs were respectively isolated, cultured and identified from human peripheral blood. EPCs from healthy donors were transfected with miR-222 mimic, mimic negative control (NC), miR-222 inhibitor, or inhibitor NC. The expression levels of ETS-1 mRNA and miR-222 were measured by qRT-PCR. The protein levels of ETS-1, Akt and p-Akt were analyzed by western blot. The migratory ability of EPCs was assessed using Transwell assay. Results MiR-222 can regulate the expression of ETS-1 mRNA and protein. Over-expression of miR-222 significantly down-regulated the expression of ETS-1 in EPCs at the mRNA and protein levels and inhibited migratory ability of EPCs (all P ＜ 0.05 ). Furthermore, knockdown of miR-222 significantly up-regulated the expression of ETS-1 in EPCs at the mRNA and protein levels and promoted migratory ability of EPCs (all P ＜ 0.05 ). Western blot showed that up- regulation of miR-222 reduced the levels of p-Akt. Down-regulation of miR-222 increased the levels of p-Akt (all P ＜ 0.05). Conclusion MiR-222 regulated the migratory ability of EPCs via its target,EST-1,and through Akt signaling pathway.
Keywords：Endothelial progenitor cells (EPCs )microRNA-222 (miR-222 )Transcription factorAkt signaling pathway
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 208-212,后插3 )