Effect of thyroxine pretreatment on tumor necrosis factor-α and lung pathology in septic rats
Zou Wen-mao
Li Jing-hui
Liu Lei
Liu Fu-rong
Deng Chao
Ao Xue
Abstract：Objective To investigate the effect of thyroxine pretreatment on tumor necrosis factor-α (TNF-α) and lung pathology in septic rats. Methods LPS (5 mg/kg) was injected into the caudal vein of rats to establish the sepsis model. Thirty-six male Wistar rats were randomly divided into blank control group (group C), sepsis group (group S) and thyroxine pretreatment group (group L),with 12 rats in each group. Group L was given by gavage with levothyroxine sodium(10 μg/100 g) for 7 days, and equivalent physiological saline were administered in the same protocol without levothyroxine sodium for 7 days in group S and group C. Rats in each group were drew blood after modeling for 0,3,6,12, 24 h. And the levels of TNF -α, free three triiodothyro (FT3) and free thyroxine (FT4) were measured. Rats were sacrificed after 24 h and lung tissue was taken to measure the wet/dry weight(W/D),and HE staining and immunohistochemistry were performed. Results The level of FT3 and FT4 was not significanlg different among each group at 0 h (P﹥0.01). However it began to decrease at 3 h,and decreased to a minimum at 6 h in group S and group L. The FT3, FT4 level in group L and group S was lower than that in group C(P<0.01),and reduced more markedly in group S (P<0.01). The W/D in group S and group L was higher than that in group C (P<0.01), and elevated more markedly in group S (P<0.01). The pathological findings showed that the alveolar structure of group C was intact. However in group S, the alveolar wall collapsed with alveolar septum broadening,and the inflammatory cell infiltration was obvious. Compared with group S,the pathological changes of group L were obviously reduced. The serum TNF-αconcentration started to elevate at 3 h, and reached a peak at 6 h in group S and group L. It was higher than that in group C(P<0.01),and elevated more markedly in group S(P<0.01). The TNF-α positive cell number and staining intensity of the lung tissue was the most and the strongest in group S, middle in group L, and the least and the weakest in group C. Conclusion Thyroxine pretreatment can decrease the level of TNF-α and improve the pathological changes of lung tissue in septic rats.
Keywords：ThyroxineSepsisNonthyroid illness syndrome (NTIS)LungTumor necrosis factor-α (TNF-α)
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 179-183 )