Protective effects of immunization with recombinant procalcitonin on sepsis - induced acute kidney injury in mouse

Li Hu; Yang Chun-hui; Cui Yu-hui; Huang Li-ou; Tang Jian-guo

doi:10.3969/j.issn.1002-1949.2018.02.008

Abstract：Objective To investigate the renal protective effects of immunization with recombinant procalcitonin (rPCT) in mouse with sepsis -induced acute renal injury, and explore its possible mechanism. Methods Thirty male Balb/c mice were randomly divided into three groups, which were sham operation group (sham group,n=10),cecal ligation and puncture (CLP group,n=10) group and immunization with rPCT group (immunization group, n = 10). Sepsis model was reproduced in mice with cecal ligation and puncture (CLP). Mice were immunized with 50 μg of recombinant procalcitonin (PCT) 3 weeks, 2 weeks and 1 week respectively before CLP operation by intraperitoneal injection. Serum creatinine (SCr), blood urea nitrogen (BUN), PCT, tumor necrosis factor-α (TNF-α),interleukin-1β and 6 (IL-1β, IL-6) were determined 24 hours after CLP operation. Morphological changes of the kidney were observed by H E staining. Results Compared with sham group, serum urea nitrogen, TNF -α, IL -1β and IL -6 were significantly high in CLP group (P<0.01). However,when compared with CLP group,SCr,BUN,TNF-α,IL-1β and IL-6 were significantly lower in immunization group. Kidney morphology in sham group was normal,while in CLP group,some pathological changes occurred,such as tubular epithelial cells swelling and exfoliation, vesicular degeneration,destruction of brush border and neutrophil infiltration of renal interstitium. These changes were ameliorated in immunization group. Kaplan-Meier survival analysis showed that 7-days mortality of mouse in immunization group was lower than in CLP group. Conclusion PCT might promote the release of inflammatory mediators in the process of sepsis induced AKI, thus aggravate the disease progression. Immunization with rPCT might play a protective role on it.