The effect and mechanism of exogenous hydrogen sulfide on atherosclerosis in apolipoprotein E-/-mice
Yang Qin
Jiang Hong
Wen Yuan
He Long-miao
Zhang Ming-xia
Huang Jun
Abstract：Objective To investigate the regulation and mechanism of exogenous hydrogen sulfide(H2S) on atherosclerosis in ApoE -/-mice by Toll-like receptor 4(TLR4).Methods The carotid artery atherosclerotic plaques in ApoE -/-mice model was established;the mice were fed with a high-fat diet and administered GYY4137 for 8 weeks.Lipid and atherosclerotic lesions were measured by oil red O staining.TNF-α, IL-6, IL-1 and IL-10 were detected by ELISA.Protein expression of TLR4, PI3K, Akt, P-Akt were evaluated by Western blot .Results Compared with the control group , the lipid composition and proinflammatory factors TNF -α, IL-6 and IL -1 increased in the model group, and the inhibitory factor IL-10 decreased, and the expression of TLR4, PI3K and P-Akt protein increased in atherosclerotic plaques (P<0.01).GYY4137 medium and high dose intervention reduced the lipid composition and proinflammatory factors TNF -α, IL-6, IL -1 expression in the model group , increased the inhibitory factor IL -10, decreased the expression of TLR 4, PI3K and P-Akt protein ( P<0.01).The effect of LY294002 intervention was similar to that of GYY4137.Conclusion Exogenous H2 S can regulate the expression of TLR 4 and TNF-α, IL-6, IL-1 and IL -10, and prevent the development of carotid atherosclerotic plaques in ApoE -/-mice, which is related to the PI3K-Akt signaling pathway .
Keywords：AtherosclerosisHydrogen sulfide ( H2 S)Inflammatory factorToll -like receptor 4( TLR4)
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 94-96,后插1 )