Vitamin D3 is involved in the regulation of pulmonary dendritic cell activity in septic rats

Sun Le-ke; Hu Lin-feng

doi:10.3969/j.issn.1002-1949.2017.12.007

Abstract：Objective To understand the mechanism of vitamin D3 (Vit D3) in the treatment of sepsis through the regulation of pulmonary dendritic cells (DCs),we examined CD83 and CD86 and anti-inflammation and proinflammatory cytokines in septic rat model.Methods ①Female juvenile rats (n =24) were randomly divided into three groups including normal control group,septic group and Vit D3 intervention group.② The rats in the septic group and Vit D3 intervention group were intraperitoneal injected with lipopolysaccharide (LPS) for preparation of septic model.③The expression of CD83 and CD86 on the surface of pulmonary DCs in the rats was detected using flow cytometry.④Proinflammatory cytokines (IL-12) and anti-inflammatory factor (IL-10) in the lung tissue of rats and 25 (OH) D3 in the rat serum were detected by ELISA.Results The expression levels of CD83 and CD86 on the surface of pulmonary DCs in the septic group (6.81 ± 0.44) ％ and (5.52 ± 0.67) ％ and Vit D3 intervention group (4.58 ± 0.47)％ and (3.19 ± 0.50)％ were significantly increased,comparing to the normal control group (0.86 ± 0.13) ％ and (0.54 ± 0.16) ％ (t =36.115,18.128 and 18.735,18.908,P ＜ 0.05),but the expression levels of CD83 and CD86 in the Vit D3 intervention group were lower than those in the septic group (t =13.326,7.433,P ＜ 0.05),respectively.The inflammatory factor IL-12 in the lung tissue in the Vit D3 intervention group (114.71 ±45.61) ng/L was reduced comparing to the septic group (343.86 ± 97.87) ng/L(t =8.595,P ＜ 0.05),whereas the anti-inflammatory factor IL-10 level in the Vit D3 intervention group (194.18 ± 24.53) ng/L was higher than that in the septic group (150.59 ± 32.48) ng/L(t =2.933,P ＜ 0.05).The serum 25 (OH) D3 level in the Vit D3 intervention group (41.76 ± 7.97) mg/L was increased compared with the septic group (21.58 ±8.04) mg/L(t =6.941,P ＜0.05),but serum 25(OH) D3 level in these two groups were significantly lower than that in the normal control group (60.24 ± 5.67)mg/L(t =8.388,10.050,P ＜ 0.05).Conclusion Vit D3 may inhibit the activity of pulmonary DCs in septic rats and alleviate the excessive immune response and inflammatory response in sepsis.