The expression of COX-2, Ca2+-ATPase and collagenation in paraquat-induced lung injury rats treated with magnesium sulfate
Li Jun-ying
Liang Zong-an
He Qing
Zheng Jun
Abstract：Objective To investigate the expressions of COX-2,Ca2 +-ATPase and collagenation in paraquat-induced lung injury in rats treated with magnesium sulfate.Methods Sixty male Sprague-Dawley rats were randomly (random number method) divided into three different groups:paraquat intoxication group (PQ group);magnesium sulfate group (MS group);controlled group (NS group).PQ group and MS group were given paraquat through intra-gastric administration (80 mg/kg).MS group was intraperitoneally administrated given douched magnesium sulfate (30 mg/kg per day),while NS group was given equivalent volume of normal saline.The rats of these three groups were further divided into four sub-groups:3 d,7 d,14 d and 21 d.The level activity of Ca2+-ATPase in lung homogenate was measured.The expressions of COX-2 and collagen fiber were detected by immunohistochemistry.Results The COX-2 expression was significantly higher in the rats lung of paraquat intoxication groups (PQ group and MS group) compared with NS group,while there was a significant difference between MS group and PQ group (P ＜ 0.05).The Ca2 +-ATPase activity in PQ group was lower than that of MS group(P ＜ 0.05).Compared with NS group,the Ca2 +-ATPase activity of PQ group was significantly different during treatment period (P ＜ 0.01),but the Ca2+-ATPase activity of MS group was significantly different on the 3rd,7th and 21 th day (P ＜ 0.05).In MS group,the Ca2+-ATPase activity increased from the 14th day and returned to normal level on the 21th day.However,the Ca2+-ATPase activity of PQ group did not increase until on the 21th day.The collagen fiber among these groups had no significant difference on the 3rd day.But a gradually increase of collagenation could be observed in PQ group on the 14th day,and it presented a strongly fibrosis.MS group had lower collagen fiber hyperplasia and structure destroy than PQ group.Conclusion Magnesium sulfate could lessen lung injury by reducing the expression of COX-2,decreasing angiogenesis and inhibiting calcium overload.
Keywords：Magnesium sulfateParaquat(PQ)Lung injuryCOX-2Ca2 +-ATPase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1045-1048,封3 )