Infulence of Neostigmine on bungarotoxin induced masthenia in rats

Chen Shao-Ying; Li Yan; Liang Zi-jing; Liang Qing; Yan Ping; Liu Rong

doi:10.3969/j.issn.1002-1949.2017.10.019

Abstract：Objective To investigate the effect of Neostigmine on myasthenia of rats which is poisoned by bungarotoxine at early stage. Methods SD rats were randomly divided into eight groups. A group was neostigmine 0. 09 mg/kg used 15 min after poisoned. B group was neostigmine 0. 09 mg/kg used 30 min after poisoned. C group was neostigmine 0. 09 mg/kg used 1. 5 h after poisoned. D group was neostigmine 0. 18 mg/kg used 15 min after poisoned. E group was neostigmine 0. 18 mg/kg used 30 min after poisoned. F group was neostigmine 0. 18 mg/kg used 1. 5 h after poisoned. G group was untreated after poisoned. H group was blank group. The rats were given celiac anaesthesia with 10%chloral hydrate 0. 03 mL/100 g, and then fixed to mounting plate to take repetitive nerve stimulation check. After given injected subcutaneously with bungarotoxin 0. 24 mg/kg, the EMG attenuation values, and partial pressure of oxygen were recorded. All of the index was statistical analyzed. The diaphragm, gastrocnemius were stained with HE. Results There was no statistically significant difference in attenuation values, and partial pressure of oxygen between the groups treated with neostigmine and the control group(P >0. 05). There was no statistically significant difference in attenuation values, and partial pressure of oxygen between the groups treated with the same dose of neostigmine at different time (P>0. 05). There was no statistically significant difference in attenuation values, and partial pressure of oxygen between the groups treated with the different dose of neostigmine at the same time(P>0. 05). Pathological examination show the cells of diaphragm and gastrocnemius muscle swelling, nuclear disorganized at high magnification. There was no statistically significant difference between the groups. Conclusion The effect of neostigmine to antagonize muscle weakness caused by bungarotoxin in poisoned rats is not observed.