The study on the longer time course of seawater aspiration-induced acute lung injury and stress response
Yuan Jia-jia
Zhang Xiao-tong
Bao Yun-ting
Xu Zheng-qing
Shu Yuan-zhao
Wang Zi-wei
Du Bin
Pang Qing-feng
Abstract：Objective To observe the morphological and biochemical index of seawater aspiration-induced acute lung injury from 15 min to 3 d after drowning. Methods A total of 84 male mice were assigned to 10 groups including normal group ( 10 ) , non -survival group ( 22 ) and near -drowning 15 min(10), 30 min(6),1 h(6), 3 h(6), 6 h(6), 12 h(6), 24 h(6), 72 h(6). Lung wet-to-dry weight ratio, blood gas and biochemical analysis were examined in the normal group, near, drowning 15 min group, and non-survival group. The computed tomography images were obtained using 64 mA 120 kV with a voxel of 1. 0 mm. The activity of superoxide dismutase, the content of methane dicarboxylic aldehyde, the content and activity of heme oxygease-1, and the content of aquaporin-1 in the lung tissue were determined. The pulmonary histological changes were observed under light microscope. Results Compared with normal group, the lung injury in near - drowning 15 min, 30 min, 1 h, 3 h, 6 h groups was more serious, especially in 1 h group. In 1 h group, the level of methane dicarboxylic aldehyde is highest while superoxide dismutase activity was lowest. The expression and enzymatic activity of heme oxygease-1 significantly increased and were sustainable for 3 d. Meanwhile, aquaporin -1 expression increased at peak in 6 h group. Conclusion The seawater aspiration -induced lung injury can self-repair partly dependent on heme oxygease-1 and aquaporin-1.
Keywords：SeawaterAcute lung injuryHeme oxygenase-1Aquaporin-1Self-repair
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 946-951 )