Protective effects of ulinastatin on blemycin A5-induced pulmonary fibrosis in rats
Li De-jun
Jin Chang-jun
Ji Hong-sheng
Zhao Bao
Xu Chun-yang
Xia Wen-jun
Han Li-hui
Abstract：Objective To investigate the therapeutic effect and mechanism of ulinastatin on blemycin A5-induced pulmonary fibrosis in rats.Methods 75 rats were divided into normal group, model group and ulinastatin group.Rat pulmonary fibrosis was induced by intrabronchial injection of blemycin A5(5 mg/kg).Treatment was started from day 2 to day 28 with ulinastatin(20 000 U/kg).The pathological changes were observed by hematoxylin and eosin(HE) staining and expressions of transforming growth factor-β1(TGF-β1) protein and tumor necrosis factor-α(TNF-α) protein were tested by immunohistochemical technique.Results The pulmonary alveolitis and fibrosis were alleviated remarkable in the lung pathological section in ulinastatin group compared with those in the model group(P<0.01).The expression of TGF-β1 protein and TNF-α protein were decreased significantly than those in the model group(P<0.01).Conclusion Ulinastatin ameliorates the pulmonary fibrosis and its mechanisms may be through decreasing the expression levels of TGF-β1 protein and TNF-α protein.
Keywords：Pulmonary fibrosisUlinastatin(UTI)Blemycin A5Transforming growth factor-β1(TGF-β1)Tumor necrosis factor-α(TNF-α)
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 827-829 )