Effects of hypoxic cultured bone marrow mesenchymal stem cells on changes of cytokines in paraquat-induced acute lung injury in rats

Huang Yang; Sun Ji-yuan; Yin Wen; Wang Yu-tong; Zhao Wei; Li Jun-jie; Chen Shi

doi:10.3969/j.issn.1002-1949.2017.08.013

Abstract：Objective To investigate the effects of hypoxic cultured bone marrow mesenchymal stem cells (BMSCs) on changes of cytokines in paraquat-induced acute lung injury in rats.Methods BMSCs were isolated from SD rats and divided into normoxic culture group(20%O2) and hypoxic culture group(5%O2).The proliferation and determination were observed and compared between the both groups.Sixty SD rats were randomly divided into four groups: a PQ group, a normoxic cultured BMSCs treatment group, ahypoxic culturedBMSCs treatment group and a normal control group.The rats′ survival time were calculated during 14 days after PQ injections in each group.At the same time, the levels of Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) in plasma were detected.Results ①The BMSCs cultured under hypoxic condition appeared differentiation partly.But most of the cells remained the characteristics of BMSCs, and the stability was good.②Compared with the normoxic cultured BMSCs, the proliferation of the hypoxic cultured BMSCs increased markedly (P≤0.05), and the numbers of colony formation increased significantly (P≤0.01).③Compared with PQ group, the rats were all alive within 14 days in normoxic cultured BMSCs treatment group and hypoxic cultured BMSCs treatment group.④Compared with normoxic cultured BMSCs treatment group during 7 days after PQ Poisoning, the levels of IL-1β and TNF-α in plasma in hypoxic cultured BMSCs treatment group were decreased significantly (P≤0.01), the levels of MDA in plasma decreased markedly (P≤0.05), and the activities of SOD, GSH-PX in plasma increased obviously (P≤0.05).Conclusion The hypoxic cultured BMSCs are more adapt to clinical pathological physiological environment than the cells cultured in normoxia, which have stronger ability of inhibiting inflammatory mediator levels, scavenging free radical and resisting lipid peroxidation injury.