Inhibitory effect of galangin on LPS-induced inflammatory reaction of macrophages
Hu Huan
Zhang Zhen-hui
Liu Ning-ning
Kong Tian-yu
Gu Hong-jiao
Xiong Xu-ming
Abstract：Objective To explore the effect of galangin on the inflammation reflection in lipopolysaccharide(LPS)-instimulated macrophages.Methods The macrophages were generated by PMA-induced THP-1 cells, then cells were pretreated with galangin at different concentrations.Cell proliferation was detected by MTS assay.Cell apoptosis was determined with Annexin V-FITC /PI double staining by flow cytometry.Inflammatory factors including TNF-α, IL-1β, IL-6, TIMP-1 were tested by ELISA.Western blot was used to analyse the protein levels of MMP-2 and MMP-9.Results Galagin within 0~20 μmol/L was not toxic to macrophages.Concentration of LPS was 500 ng/mL.Compared with the group treated with LPS alone, galangin within 2.5~20 μmol/L could inhibit the expression of TNF-α, IL-1β, IL-6 (P<0.05), but had no effect on the expression of TIMP-1.Galangin within 10~20 μmol/L could suppress the levels of MMP-2 and MMP-9.Conclusion Galangin decreased the levels of inflammatory factor including TNF-α, IL-1β, IL-6, MMP-2, MMP-9 in LPS-induced macrophages.
Keywords：GalanginMacrophagesLipopolysaccharide(LPS)Inflammatory factorMMPs
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 555-559,后插4 )