Brain injury after cardiopulmonary resuscitation from cardiac arrest in rats induced by three dif-ferent methods
Jing Ying-xia
Li Xiang-min
Abstract：Objective To determine the brain injury degree of rats by comparing the expres-sions of ICAM-1 and VCAM-1 in brain tissue after cardiopulmonary resuscitation from cardiac arrest induced by three different methods. Methods Cardiac arrest animal models of rats were built with the use of three methods including suffocation, chest oppression with 3-kilogram object, and clamping of cardiac plexus. Three groups of rats were respectively sacrificed 3, 6 and 12 hours after resuscitation. The cerebral cortical tissues were harvested to determine ICAM-1 and VCAM-1 expression by immu-nohistochemical methods. Results Compared with the control group, ICAM-1 and VCAM -1 ex-pressions at the corresponding time points in the experimental groups were significantly increased(P<0.05), but the differences in ICAM-1 and VCAM-1 expression among the experimental groups were not significant(P>0.05). ICAM-1 and VCAM-1 contents in brain tissue of rats sacrificed 6 h and 12 h after resuscitation from cardiac arrest induced by chest oppression with 3-kilogram were higher than in rats sacrificed 3 h after resuscitation(P<0.05);for rats resuscitated from cardiac arrest induced by the clamping of cardiac plexus, ICAM-1 expressions in brain tissue of rats sacrificed 6 h and 12 h after resuscitation were higher than in rats sacrificed 3 h after resuscitation(P<0.05). Conclusion The degree of brain injury caused by three different methods are the basically same. All the three models built by different cardiac arrest-inducing methods can be used for the research on brain injury after cardiopul-monary resuscitation.
Keywords：Cardiopulmonary resuscitation(CPR)Animal modelsBrain injuryIn-ter-cullular adhesion molecular-1(ICAM-1)Vascular cell adhesion mo-lecular(VCAM-1)
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 365-368,284 )